Search for projects
Daylily (Hemerocallis L.). A New Method of Breeding and Cultivation
Over the last 10 years daylily breeding has reached its peak, however, the majority of daylily varieties are created in the U.S.A., thus making them barely resistant to the Lithuanian climate. Hence, I decided to create a new variety of daylilies resistant to the climate of Lithuania. To fasten the process, I decided to grow the seedlings indoors, which shortens the bloom time to 9 months. I investigated 3 main factors: temperature, pollination time and ploidy (tetraploid or diploid), and found that the best time for crossing daylilies is 10 a.m., the most efficient temperature is 18-20 °C, and the diploids set pods better than tetraploids. The main result is a new variety of daylily – Hemerocallis ‘Mitsu’, which I tested for 3 years, and it grows well after winter.
Antibacterial substances in clothes - do they fulfil their purpose?
Martin Göök Johansson
During recent years, the usage of several domestic antibacterial substances has increased. Triclosan is such a substance and it has also proven to be a toxic substance. Triclosan has also known to cause long term catastrophic effects on the marine environment and can potentially disrupt the endocrine system and the reproduction of marine species. It can also disrupt the reproduction North American bullfrogs. The purpose of this study has been to find out whether Triclosan, has any antibacterial effect in socks. I have also found out if Triclosans antibacterial properties are reduced after wash. My results indicate that that Triclosan does not have any significant antibacterial effect in socks, compared to untreated socks. In my paper I discuss whether Triclosan can be removed from the market.
A statistical investigation of the effects of diazotroph bacteria on plant germination
Ciara Judge, Emer Hickey, Sophie Healy-Thow
Diazotroph bacteria are well known to have a symbiotic relationship with the legume plant family whereby the bacteria thrive while providing energy to the plant itself via the nitrogen fixation process. The project outlines an extensive experimental program to assess the impact of such bacteria on non legume crop species. The results show that two types of the rhizobium family of Diazotroph bacteria produce a statistically significant acceleration ( increase of 17% for Rhizobium leguminosarum and 28% for Rhizobium japonicum; p<001 ) in the rate of barley crop germination. The results are based on an analysis of the performance of over 5,290 seed samples in 105 experimental runs over a 6 month period. In barley field trials, Rhizobium japonicum bacteria inoculated at 6x106cfu/ seed also increased growth rates and gave a 13.2% (p=0.0328) dry mass yield improvement. The studies have significant potential for improving the productivity of valuable food crops by increasing yields, reducing fertiliser use, and by reducing losses due to disease and weather. Opportunities to commercially scale up bacterial treatment of seeds treatment are being investigated. Investigations continue into the application of diazotroph bacteria in the germination phase of the malting process in brewing.
Sequencing of LEPREL1 Gene in Myopic Patient Family
Myopia or near-sightedness is one of the most common eye diseases in the world. Although it can be easily corrected with the help of corrective lenses such as glasses or contact lenses, such vision enhancement methods are uncomfortable as they are not permanent. The only way to permanently enhance vision is surgical intervention, which is expensive and can cause side effects. Like other hereditary disorders, myopia is induced by alterations in genetic material. Therefore it is important that the genes causing myopia are found and examined, so that people who are carriers of these alterations can be made aware about the condition and undertake precautionary actions to hinder the development of myopia. At present there is only one known gene – LEPREL1, alterations of which cause nonsyndromic myopia. In this research paper the author has analysed the nucleotide sequence of LERPEL1 gene exons in a nonsyndromic myopia patient family and has found a nucleotide variation that may cause myopia.
Occurrence of Branchiobdellid worms (Annelida: Branchiobdellidae) on the stone crayfish (Austropotamobius torretium) in the south-west part of the Czech Republic
Klára Nováková, Kateřina Fialová
The aim of this work is to map the occurrence of Branchiobdella species on the stone crayfish (A. torrentium) and to investigate the basic population characteristics (abundance and length structure) of Branchiobdellid worms in 5 localities of Pilsen region. Specimens were taken from caught crayfish and then determined on the base of morfological features of body, jaws and spermatheca using an optical microscope. Five species of Branchiobdella were found. They are Branchiobdella parasita, B. astaci, B. italica, B. hexodonta and B. pentodonta. All results were compared with already existing data of the occurrence of Branchiobdellid worms in the Czech Republic and Europe.
THE EFFECT OF ANKAFERD AND L-CARNITINE ON ANGIOGENESIS IN CHORIOALLONTOIC MEMBRANE (CAM)
Mert GÜLŞEN, Nur Uzunay
Angiogenesis is needed for tumor growth and metastasis. For this reason, it represents an exciting target for cancer treatment. Some anti-angiogenic drugs may be useful to prevent angiogenesis during cancer treatment. The aim of this study is to investigate if Ankaferd(standardized herbal extract obtained from five different plants namely Thymus vulgaris,Glycyrrhiza glabra, Vitis vinifera, Alpina officinarum,and Urtica dioica) and L-Carnitine (a quaternary ammonium compound biosynthesized from the amino acids lysine and methionine) have an effect on angiogenesis in chick embryo chorioallontoic membrane. Ankaferd was applied at 1,5,20 ,50% and L-Carnitine 390 mmol/L in concentration to the fertilized eggs on the 6th day. Physiologic serum was used for control group. At the end of the study (day 7 and 8), all eggs were opened and vessels were examined by magnifying glass. No significant changes were observed in 1% Ankaferd group and control group. But significant inhibition in angiogenesis was observed in 5,20,50% Ankaferd groups. In L-Carnitine group, angiogenic effect was marked. In conclusion, the anti-angiogenic effect of Ankaferd was shown experimentally in this study (first time in the medical literature). So, Ankaferd may be an antineoplastic drug in the future. According to our results, L-Carnitine has angiogenic effect. Due to this effect, it may be beneficial in the treatment of ischaemic disease and endothelial dysfunction. Although these results are promising, large-scale, prospective studies are needed in humans to provide more information about these drugs.
Honey bee's (Apis mellifera L.) foraging range based on panlynological analisis of the composition of its pollen loads
Minute bees - great ventures (Honey bee's (Apis mellifera L.) foraging range based on panlynological analisis of the composition of its pollen loads): My study of honey bees foraging range was based on a self made, innovative method. The proposed method uses simple, cheap and, imprimis, harmless tools. My method starts with the permisses, that:
- honey bees polinate flowers and take pollen from indicative plants
- bees form pollen loads, where the wanted pollen is located
- they always return home
We are able to take from bees their pollen loads (and then analyse them) I found a few unitary species, e.g. two sweet chestnuts (marrons), two manna ashes and three buckwheat fields. Once a week (during 16 weeks), I took pollen loads from pollen trap located in Langsroth‘s beehive. The collected material was stored properly, whereupon microscopic slides were compiled and then submitted for palynological analysis. It was a must to analyze 300 pollen grains every week, what eventually enabled me to:
- examine honey bees foraging range
- compose their „menu“ during sixteen weeks My method is:
- safe for bees
- relatively cheap and easy to do (there is no need to involve the whole research team)
- enables us to examine Apinae‘s (inter alia bumblebees and western honey bees) and Osmia rufa‘s foraging range
- futhermore, it enables beekeepers to locate their apiary properly and obtain the aimed apian products Let me draw your attention to these tiny creatures, older than mankind. They live in terrific, well-ordered communities and they are extremly salutary to us. I would like mankind to focus on requiting them, e.g. without devouring their populations by enviromental contamination or supermanuring plant fields.
Molecular aspects of nitrogen excess on wheat plants in the vegetative stage
Increased content of inorganic nitrogen in soil is responsible not only for various ecological problems, but also induces stress to plants. In other hand, in some environmental or biotic stresses (pathogens) is it’s consumption increased over the optimal level of unstressed plants because of high energetic costs of defensive responses (synthesis of defensive proteins and other nitrogenous compounds). Unlike physiology of insufficient nitrogen nutrition or physiology of various stresses is physiology of excess nitrogen nutrition very poorly investigated. Objective of our work was to investigate impact of high nitrogen dosages in form of ammonium nitrate to plants of wheat in vegetative stage in ideal growth conditions. Our applied dosages of inorganic nitrogen in watering solutions (7 – 35 mM) induced inhibited growth and production of biomass, thinning of stems and decrease of count and total area of stomata in the epidermis of the leafs. Plants stressed by excess nitrogen in soil in our experiments in increased rate accumulated chlorophylls, free proline and soluble proteins, mostly small and large subunit of enzyme RuBisCO. Activity of isoforms of glucanase with lower molecular weights (30 kDa and 38 – 40 kDa) was impaired, rather than activity of heavier isoform (49 – 52 kDa) was stimulated. With our work we contributed to extension of still poor knowledge of physiology of plants in condition of excess nitrogen supply. Results can be used in further experiments leading to improvement of management of nitrogenous fertilization, especially in conditions of various stresses.
Plants in Agarose Gel
Jasper D'Eer, Jonas Borgmans
Our project is, like the title already states, about plants in gel. A combination which most of us already now, but there’s a big difference between our gel and the gel you can buy at your local garden supplier. The gel you get from your retailer doesn’t give any nutrition to your plants, it only gives water and support. We researched if you could use a gel which contains nutrition to grow plants. For this gel we made use of agar (more purified form = agarose), this product is used as a substitute for gelatin and is used in biological labs. People could use this instead of natural soil when for example their soil is polluted, isn’t solid enough, can’t hold enough water,…
Illumination of Cancer Cells by Fluorescent Carbon Nanotubes
Ege Salman, Ecem Eker
In this study, cyan fluorescent protein (CFP) was chosen as fluorescent probe for cancer cell imaging. CFP was noncovalently (adsorption) conjugated to acid-treated multiwalled carbon nanotubes (MWCNT). The obtained f-MWCNT/CFP conjugates were then linked to folic acid via –NH3 ends of proteins. The application potential of the obtained bioconjugates was investigated using HeLa cell line and A549 cell line which were chosen as model cells. In vitro fluorescence microscope images show that these conjugates can be used as an efficient fluorescent probe for targeting and imaging of folic acid over expressed cancer cells.